Column Chromatography

Column Chromatography is a common and useful separation technique in organic chemistry. This separation method involves the same principles as TLC, but can be applied to separate larger quantities than TLC. Column chromatography can be used on both a large and small scale. It is a technique used to separate the components of a chemical mixture by moving the mixture along a stationary material, such as gelatin.

Different components of the mixture are caught by the material at different rates and form isolated bands that can then be analyzed. Stationary phases for CC can come in a variety of sizes, activities, acidic and basic variations for both alumina and silica. Column chromatography is a commonly used technique for protein purification.

Column chromatographic methods are generally more complicated to set up than batch methods such as ammonium sulfate precipitation; however, they are capable of high resolution separation and are usually necessary for a successful purification. Like other separation techniques, different column chromatography methods exploit different properties of the protein. Ion exchange chromatography separates molecules based on charge.

Sorbead India is an endeavor which manufactures various grades of silica and alumina for chromatographic separations, especially for column chromatography. This endeavor measures the pore diameter using the BET method which guarantees the best and most accurate results during manufacturing process. Sorbead India offers products of various pore diameters for a range of different particle sizes, required for complex chromatographic separation processes. The applications of this technique are wide reaching and cross many disciplines including biology, biochemistry, microbiology and medicine. The technique is specially used in laboratories and pharmaceuticals and many common antibiotics are purified by this procedure.


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